Here are the celebrity guests who made an appearance on 25 Words or Less.
- Cristela Alonzo
- Tim Bagley
- Lance Bass
- Betsy Brandt
- Dan Bucatinsky
- Tisha Campbell
- D'Arcy Carden
- Gabrielle Carteris
- Courteney Cox
- Margaret Cho
- Kim Coles
- Marcia Cross
- Wilson Cruz
- Whitney Cummings
- Taye Diggs
- Illeana Douglas
- Colton Dunn
- Bill Engvall
- Patrick Fabian
- Joey Fatone
- Trinity Fatu
- Kate Flannery
- Dave Foley
- Rick Fox
- Amanda Fuller
- Ron Funches
- Willie Garson
- Peri Gilpin
- Robin Givens
- Brad Goreski
- Greg Grunberg
- Beth Hall
- Shanola Hampton
- Chelsea Handler
- John Michael Higgins
- Dulé Hill
- Pete Holmes
- Matt Iseman
- Penn Jillette
- Chris Kattan
- Jamie Kennedy
- Carson Kressley
- Lisa Kudrow
- Lauren Lapkus
- Carol Leifer
- Colby Lopez
- Loni Love
- Jane Lynch
- Joshua Malina
- Debbie Matenopoulos
- Ross Mathews
- Mary McCormack
- Heather McDonald
- Michael McDonald
- Joel McHale
- Camryn Manheim
- Debra Messing
- Finesse Mitchell
- Natalie Morales
- Elise Neal
- Natalie Neidhart
- Chris Noth
- Oscar Nuñez
- Robert O'Mara
- Ana Ortiz
- Gary Owen
- Melissa Peterman
- Russell Peters
- Drew Pinsky
- Teri Polo
- Lindsay Price
- Greg Proops
- Missi Pyle
- Rebecca Quin
- Mary Lynn Rajskub
- June Diane Raphael
- Caroline Rhea
- Melissa Rivers
- James Rodriguez
- Russell Rope
- Sara Rue
- Gabrielle Ruiz
- Roselyn Sanchez
- Nico Santos
- Jamie-Lynn Sigler
- Mira Sorvino
- Hal Sparks
- Amber Stevens West
- Curtis Stone
- Nicole Sullivan
- Alison Sweeney
- Raven-Symoné
- Lea Thompson
- Jackie Tohn
- Lynn Toler
- Jessica Tuck
- Sesugh Uhaa
- Sheryl Underwood
- James Van Der Beek
- Lisa Ann Walter
- Julie Warner
- Steven Weber
- Jaleel White
- Bradley Whitford
- Kym Whitley
- Marissa Jaret Winokur
- Eric Winter
- Josh Wolf
- Chely Wright
- Noah Wyle
- Cedric Yarbrough
- Sasheer Zamata
- Constance Zimmer
- Daphne Zuniga